OnePCR is an all-in-one, fully automated PCR detection system that offers a streamlined sample-to-result workflow, eliminating the need for sample transfer between instruments, which reduces the turnaround time and minimizes the risk of human errors. This highly sensitive, comprehensive solution provides reliable detection using automated magnetic bead-based nucleic acid extraction, followed by RT-PCR detection in a single run. It utilizes an innovative single-use disposable sealed extraction and PCR cartridge to avoid contact with the instrument, environment, or any carryover contamination.