The KRONUS Insulin Autoantibody (IAA) RIA Assay Kit is for the semi-quantitative determination of antibodies to insulin in human serum, and is useful as an aid in the diagnosis of Type 1 diabetes mellitus (autoimmune mediated diabetes) in patients who have not received insulin therapy. As the KRONUS IAA RIA assay kit does not discriminate between insulin autoantibodies and antibodies produced in response to exogenous insulin therapy, measurement of autoantibodies to insulin must be done prior to insulin therapy. Once insulin therapy has begun, antibodies may form against the therapeutic insulin that will be detected by the KRONUS IAA RIA assay.